Human Phospho-PDGF R alpha (Y742) Antibody

Name: Human Phospho-PDGF R alpha (Y742) Antibody
Brand: R&D Systems
SKU: AF2114

Catalog #: AF2114
3 Citations Datasheet / COA / SDS

Discontinued Product

AF2114 has been discontinued.

View all PDGF R alpha products.

Detection of Human Phospho-PDGF R alpha (Y742) by Western Blot.

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Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-PDGF R alpha (Y742) Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF R alpha when phosphorylated at Y742.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human PDGF R alpha Y742 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-PDGF R alpha (Y742) by Western Blot. Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-PDGF Ra (Y742) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2114), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF Ra (Y742) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References

Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.

Long Name

Platelet-derived Growth Factor Receptor alpha

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Alternate Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-PDGF R alpha (Y742) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2alpha-dependent translation initiation for replication and oncogenesis
Authors: O Méndez-Sol, M Bendjennat, J Naipauer, PR Theodoridi, JJD Ho, RE Verdun, JM Hare, E Cesarman, S Lee, EA Mesri
Cell Reports, 2021-12-28;37(13):110144.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
PLoS Pathog., 2017-04-12;13(4):e1006281.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
Biochem. J., 2010-03-15;427(1):29-40.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development