Human Phospho-SMC1 (S966) Antibody

Catalog # Availability Size / Price Qty
AF2677
AF2677-SP
Detection of Human Phospho-SMC1 (S966) by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Phospho-SMC1 (S966) Antibody Summary

Species Reactivity
Human
Specificity
Detects human SMC1 when phosphorylated at S966.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human SMC1 S966 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-SMC1 (S966) by Western Blot. View Larger

Detection of Human Phospho-SMC1 (S966) by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 4 hours. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-SMC1 (S966) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2677) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-SMC1 (S966) was detected at approximately ??? kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for ???time. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMC1

The ATM protein kinase phosphorylates SMC1 protein in cells exposed to genotoxins. Overexpression of a non-phosphorylatable mutant of SMC1 subjects a cell to a defective S phase checkpoint and decreased cell survival in response to genotoxic stress. In cells expressing this mutant, BRCA1 and Nbs1 are still phosphorylated, suggesting that SMC1 phosphorylation is downstream of these other phosphorylation events.

Long Name
Structural Maintenance of Chromosomes 1
Entrez Gene IDs
8243 (Human); 63996 (Rat)
Alternate Names
CDLS2; DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast); KIAA0178DKFZp686L19178; SB1.8; SB1.8MGC138332; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1; SMC1A; SMC-1A; SMC-1-alpha; SMC1L1; SMC1SMC1alpha; Smcb; structural maintenance of chromosomes 1A; structural maintenance of chromosomes protein 1A

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Phospho-SMC1 (S966) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-SMC1 (S966) Antibody and earn rewards!

Have you used Human Phospho-SMC1 (S966) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review