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Human Phospho-SMC1 (S966) Antibody

Catalog #: AF2677 Datasheet / COA / SDS

Discontinued Product

AF2677 has been discontinued.
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Detection of Human Phospho-SMC1 (S966) by Western Blot.
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Human Phospho-SMC1 (S966) Antibody Summary

Species Reactivity
Human
Specificity
Detects human SMC1 when phosphorylated at S966.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human SMC1 S966 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-SMC1 (S966) antibody by Western Blot. View Larger

Detection of Human Phospho-SMC1 (S966) by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 4 hours. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-SMC1 (S966) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2677) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band for Phospho-SMC1 (S966) was detected at approximately 145 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMC1

The ATM protein kinase phosphorylates the structural maintenance of chromosomes (SMC1) protein in cells exposed to genotoxins. Overexpression of a non‑phosphorylatable mutant of SMC1 subjects a cell to a defective S phase checkpoint and decreased cell survival in response to genotoxic stress. In cells expressing this mutant SMC1, BRCA1 and Nbs1 are still phosphorylated, a fact that suggests SMC1 phosphorylation is downstream of these other phosphorylation events.

Long Name
Structural Maintenance of Chromosomes 1
Entrez Gene IDs
8243 (Human); 63996 (Rat)
Alternate Names
CDLS2; DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast); KIAA0178DKFZp686L19178; SB1.8; SB1.8MGC138332; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1; SMC1A; SMC-1A; SMC-1-alpha; SMC1L1; SMC1SMC1alpha; Smcb; structural maintenance of chromosomes 1A; structural maintenance of chromosomes protein 1A

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