Human Phospho-SMC1 (S966) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Phospho-SMC1 (S966) by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 4 hours. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-SMC1 (S966) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2677) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-SMC1 (S966) was detected at approximately ??? kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for ???time. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The ATM protein kinase phosphorylates the structural maintenance of chromosomes (SMC1) protein in cells exposed to genotoxins. Overexpression of a non‑phosphorylatable mutant of SMC1 subjects a cell to a defective S phase checkpoint and decreased cell survival in response to genotoxic stress. In cells expressing this mutant SMC1, BRCA1 and Nbs1 are still phosphorylated, a fact that suggests SMC1 phosphorylation is downstream of these other phosphorylation events.
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