Human Phospho-SMC1 (S966) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Phospho-SMC1 (S966) by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 4 hours. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-SMC1 (S966) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2677) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-SMC1 (S966) was detected at approximately ??? kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for ???time. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The ATM protein kinase phosphorylates SMC1 protein in cells exposed to genotoxins. Overexpression of a non-phosphorylatable mutant of SMC1 subjects a cell to a defective S phase checkpoint and decreased cell survival in response to genotoxic stress. In cells expressing this mutant, BRCA1 and Nbs1 are still phosphorylated, suggesting that SMC1 phosphorylation is downstream of these other phosphorylation events.
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