Human Phospho-TOR (S2448) DuoSet IC ELISA
Human Phospho-TOR (S2448) DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
The mammalian Target of Rapamycin (mTOR) is a member of the PI 3-kinase-related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and is a promising anti-cancer agent. mTOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.
Citations for Human Phospho-TOR (S2448) DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism
Proc Natl Acad Sci USA, 2016-09-23;0(0):.
Sample Types: Cell Lysates
Targeting mTOR and AREG with everolimus, sunitinib and sorafenib in HPV-positive and -negative SCC.
Authors: Aderhold C, Faber A, Umbreit C, Birk R, Weiss C, Sommer J, Hormann K, Schultz J
Anticancer Res, 2015-04-01;35(4):1951-9.
Sample Types: Cell Lysates
Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis.
Authors: Holenya P, Kitanovic I, Heigwer F, Wolfl S
Sample Types: Recombinant Protein
No product specific FAQs exist for this product, however you mayView all ELISA FAQs
Reviews for Human Phospho-TOR (S2448) DuoSet IC ELISA
There are currently no reviews for this product. Be the first to review Human Phospho-TOR (S2448) DuoSet IC ELISA and earn rewards!
Have you used Human Phospho-TOR (S2448) DuoSet IC ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image