Human PILR-beta Alexa Fluor® 488-conjugated Antibody

(7 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human PILR-beta in direct ELISAs and Western blots. In Western blots, approximately 10% cross reactivity with recombinant human PILR-alpha is observed and less than 5% cross-reactivity with recombinant mouse (rm) PILR-beta, rmPILR-alpha, and rmPILR-L is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PILR-beta isoform 1
    Gln20-Ala189
    Accession # Q9UKJ0
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PILR‑ beta in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR‑ beta Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB4189G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PILR-beta

Paired immunoglobulin-like, type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. It is the activating counterpart to the ITIM-bearing PILR-alpha inhibitory receptor. PILR-beta is expressed in a wide variety of hematopoietic cells, including NK cells, macrophages, dendritic cells and neutrophils. Mature human PILR-beta is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and a positively-charged residues in its TM domain that interacts with ITAM‑bearing adaptor molecules. Over aa 20-189, in their ECD, human PILR-beta and PILR-alpha share 82% aa sequence identity. The aa sequence of mouse PILR-beta ECD is only 43% identical to that of human PILR-beta ECD.

  • Long Name:
    Paired-Ig-likeType 2 Receptor beta
  • Entrez Gene IDs:
    29990 (Human); 170741 (Mouse)
  • Alternate Names:
    activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC016G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 7 reviews)

Have you used Human PILR-beta Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human PILR-beta Alexa Fluor® 488-conjugated Antibody.

We have 7 reviews tested in 1 species: Human.
We have 7 review tested in 1 application: Flow Cytometry.

Filter by Ratings
Excellent
(7)
All Ratings
Filter by Applications
Flow
(7)
All Applications
Filter by Species
Human
(7)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Human Anonymous 11/27/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedNatural killer cells
SpeciesHuman
  Excellent
 Flow Human Anonymous 11/27/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedHuman NK Cells
SpeciesHuman
  Excellent
 Flow Human Anonymous 11/18/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman
  Excellent
 Flow Human Anonymous 11/10/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman
Flow Cytometry Human PILR-beta  Alexa Fluor® 488-conjugated Antibody FAB4189G
Enlarge
Excellent
 Flow Human Anonymous 10/02/2017
Show
Image Details
Flow Cytometry Human PILR-beta  Alexa Fluor® 488-conjugated Antibody FAB4189G
Flow Cytometry: Human PILR-beta Alexa Fluor® 488-conjugated Antibody [FAB4189G]

Summary

ApplicationFlow Cytometry
Sample TestedJurkat human acute T cell leukemia cell line
SpeciesHuman
Flow Cytometry Human PILR-beta  Alexa Fluor® 488-conjugated Antibody FAB4189G
Enlarge
Excellent
 Flow Human Anonymous 09/25/2017
Show
Image Details
Flow Cytometry Human PILR-beta  Alexa Fluor® 488-conjugated Antibody FAB4189G
Flow Cytometry: Human PILR-beta Alexa Fluor® 488-conjugated Antibody [FAB4189G]

Summary

ApplicationFlow Cytometry
Sample TestedB cell enriched peripheral blood mononuclear cells (PBMC)
SpeciesHuman
Flow Cytometry Human PILR-beta  Alexa Fluor® 488-conjugated Antibody FAB4189G
Enlarge
Excellent
 Flow Human Anonymous 09/23/2017
Show
Image Details
Flow Cytometry Human PILR-beta  Alexa Fluor® 488-conjugated Antibody FAB4189G
Flow Cytometry: Human PILR-beta Alexa Fluor® 488-conjugated Antibody [FAB4189G]

Summary

ApplicationFlow Cytometry
Sample TestedB cell enriched peripheral blood mononuclear cells (PBMC)
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information