Human Plexin C1 Antibody Summary
10% cross‑reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, rmPlexin A3, and recombinant human (rh) Plexin B1 is observed, and less than 1% cross‑reactivity with rhPlexin D1 is observed.
Accession # O60486
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Plexin C1 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human Plexin C1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3887, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin C1
Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the Plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine‑rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The human Plexin C1 ECD shares 85%, 82% and 71% aa identity with murine, bovine and opossum Plexin C1, respectively. Plexin C1 is widely expressed in neuronal and non-neuronal fetal and adult tissues (3). In neuronal development, its role is unclear. Semaphorin-7a (Sema 7A, CD108) binds Plexin C1 in vitro, and the two show a similar expression pattern during rat neuronal development. However, in rat, beta 1 integrins rather than Plexin C1 appear to mediate Sema 7A effects on axon outgrowth (4, 5). Plexin C1 does appear to play a role in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (6). In the immune system, effects of Sema 7A on T cell-mediated inflammatory responses also appear to be mediated by beta 1 integrins rather than plexins (7). However, Plexin C1 may function to oppose the effect of beta 1 integrins, as it does on Sema 7A-mediated spreading and dendrite formation in melanocytes (8). Plexin C1 is the receptor for the poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis by A39R (2, 9, 10).
- Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
- Comeau, M.R. et al. (1998) Immunity 8:473.
- Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
- Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
- Pasterkamp, R.J. et al. (2003) Nature 424:398.
- Xu, C. and C-M. Fan (2007) Mol. Endocrinol. 21:1234.
- Suzuki, K. et al. (2007) Nature 446:680.
- Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
- Walzer, T. et al. (2005) J. Immunol. 174:51.
- Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.
Citation for Human Plexin C1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Endothelial Semaphorin 7A promotes neutrophil migration during hypoxia.
Authors: Morote-Garcia J, Napiwotzky D, Kohler D, Rosenberger P
Proc Natl Acad Sci U S A, 2012;109(35):14146-51.
Sample Types: Whole Cells
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