Pluripotent stem cells encompass both embryonic stem cells and induced pluripotent stem cells, which are non-pluripotent cells that have been reprogrammed to a pluripotent state. These cells provide much promise for the generation of specialized cells for use in regenerative medicine. In addition, these cells are an important tool for understanding developmental and disease mechanisms.
The defining characteristics of pluripotent stem cells are their ability to self renew and to differentiate into each of the three germ layers. In order to determine if a cell is truly a pluripotent stem cell, it is important to assess these characteristics. The Human Pluripotent Stem Cell Functional Identification Kit (Catalog # SC027) contains specially formulated media supplements and growth factors that can be used to differentiate human pluripotent stem cells into endoderm, ectoderm, and mesoderm. An antibody to characterize each of the three cell types is also included; SOX17 (endoderm), Otx2 (ectoderm), and Brachyury (mesoderm). The quantity of each component in this kit is sufficient to make 200 mL of medium for differentiation. This is enough medium for the differentiation of one 24-well plate of each cell type.
Differentiation Base Media Supplement (10X)
Endoderm, Ectoderm, and Mesoderm Differentiation Supplements
Endoderm, Ectoderm, and Mesoderm Differentiation of Human Pluripotent Stem Cells. BG01V human embryonic stem cells were differentiated to endoderm, ectoderm, and mesoderm using the media supplements included in the Human Pluripotent Stem Cell Functional Identification Kit (Catalog # SC027). The kit also contains SOX17 (endoderm), Otx2 (ectoderm), and Brachyury (mesoderm) antibodies for the confirmation of differentiation status.