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Human Podocan Antibody

Catalog #: MAB4220
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB4220 has been discontinued.
View all Podocan products.
Detection of Podocan in A549 Human Cell Line by Flow Cytometry.
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Citations (1)
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Human Podocan Antibody Summary

Species Reactivity
Human
Specificity
Detects human Podocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Podocan is observed.
Source
Monoclonal Mouse IgG2A Clone # 390102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podocan
Val73-Arg661
Accession # AAH30608
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Podocan
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Podocan antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Podocan in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line were stained with Mouse Anti-Human Podocan Monoclonal Antibody (Catalog # MAB4220, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # NL008).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocan

Podocan is a 70 kDa secreted glycoprotein belonging to the small leucine-rich repeat (SLR) protein family. It lacks a serine/glycine dipeptide consensus sequence required for glycosaminoglycan attachment and is not a proteoglycan. Podocan transcripts were originally isolated from kidney podocytes. Based on EST counts, Podocan is also expressed in multiple tissues, including pineal gland, bladder and vascular tissues. Podocan binds type I collagen. The amino acid sequences of mature human and mouse Podocan share 92% identity.

Entrez Gene IDs
127435 (Human); 242608 (Mouse)
Alternate Names
PCAN; PODN; podocan proteoglycan; Podocan; SLRR5A; SLRR5AMGC24995

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Citation for Human Podocan Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proteomics characterization of extracellular space components in the human aorta.
    Authors: Didangelos A, Yin X, Mandal K
    Mol. Cell Proteomics, 2010-06-15;9(9):2048-62.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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