Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 453709
View all PON2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PON2
Ala30-Leu354
Accession # NP_000296

Specificity

Detects endogenous human PON2 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human (rh) PON1 or rhPON3.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human PON2 Antibody

Detection of Human PON2 antibody by Western Blot.

Detection of Human PON2 by Western Blot.

Western blot shows lysates of human liver tissue, Huh-7 human hepatoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Monoclonal Antibody (Catalog # MAB4344) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON2 at approximately 40-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human PON2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human liver tissue, Huh-7 human hepatoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PON2

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 2 (PON2) is a 354 amino acid, 39 kDa protein that is widely expressed in a variety of tissues and may act as a cellular antioxidant, protecting cells from oxidative stress. PON2 has arylesterase and aryldialkylphosphatase activity (EC 3.1.1.2 and EC 3.1.8.1) and can hydrolyze a number of organophosphate substrates and aromatic carboxylic acid esters. PON2 is membrane-bound and has several potential glycosylation sites. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON2 is not associated with HDL but can prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name

Paraoxonase 2

Alternate Names

A-esterase 2, Serum Aryldialkylphosphatase 2

Entrez Gene IDs

5445 (Human); 330260 (Mouse); 296851 (Rat)

Gene Symbol

PON2

UniProt

NP_000296

Additional PON2 Products

Product Documents for Human PON2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PON2 Antibody

For research use only

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