Human PON2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human PON2 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human (rh) PON1 or rhPON3.
  • Source
    Monoclonal Rat IgG2A Clone # 453709
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PON2
    Ala30-Leu354
    Accession # NP_000296
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PON2 by Western Blot. Western blot shows lysates of human liver tissue, Huh‑7 human hepatoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Monoclonal Antibody (Catalog # MAB4344) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON2 at approximately 40-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON2
The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 2 (PON2) is a 354 amino acid, 39 kDa protein that is widely expressed in a variety of tissues and may act as a cellular antioxidant, protecting cells from oxidative stress. PON2 has arylesterase and aryldialkylphosphatase activity (EC 3.1.1.2 and EC 3.1.8.1) and can hydrolyze a number of organophosphate substrates and aromatic carboxylic acid esters. PON2 is membrane-bound and has several potential glycosylation sites. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON2 is not associated with HDL but can prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL. Alternatively spliced transcript variants encoding different isoforms have been described.
  • Long Name:
    Paraoxonase 2
  • Entrez Gene IDs:
    5445 (Human); 330260 (Mouse); 296851 (Rat)
  • Alternate Names:
    A-esterase 2; Aromatic esterase 2; EC 3.1.1.2; EC 3.1.1.81; paraoxonase 2; paraoxonase nirs; PON 2; PON2; Serum Aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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