Human POT1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human POT1 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human POT1 Antibody Summary

Species Reactivity
Detects endogenous human POT1 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human POT1
Accession # Q9NUX5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human POT1 antibody by Western Blot. View Larger

Detection of Human POT1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human POT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POT1 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POT1

POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins. It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes. This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs. Human POT1 is 634 amino acids (aa) in length. It contains two N-terminal DNA binding OB regions (aa 1‑300) and a C-terminal TPP-1 dimerization domain (aa 301‑634). There are multiple isoform variants. There is a potential alternate start site at Met132, plus a three aa substitution for aa 43‑634, a 24 aa substitution for aa 317‑634, a three aa substitution for aa 457‑634, and a 16 aa substitution for aa 503‑634. Over aa 159‑316, human POT1 shares 71% aa identity with mouse POT1.

Long Name
Protection of Telomeres Protein 1
Entrez Gene IDs
25913 (Human); 101185 (Mouse); 500054 (Rat)
Alternate Names
DKFZp586D211; HPOT1; POT1 protection of telomeres 1 homolog; POT1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog (S. pombe); protection of telomeres protein 1

Product Datasheets

You must select a language.


Citation for Human POT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of TRF2 accelerates telomere attrition and DNA damage in na�ve CD4 T cells during HCV infection
    Authors: LN Nguyen, J Zhao, D Cao, X Dang, L Wang, J Lian, Y Zhang, Z Jia, XY Wu, Z Morrison, Q Xie, Y Ji, Z Zhang, M El Gazzar, S Ning, JP Moorman, ZQ Yao
    Cell Death Dis, 2018;9(9):900.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human POT1 Antibody

There are currently no reviews for this product. Be the first to review Human POT1 Antibody and earn rewards!

Have you used Human POT1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review