Detection of Human POT1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human POT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POT1 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins. It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes. This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs. Human POT1 is 634 amino acids (aa) in length. It contains two N-terminal DNA binding OB regions (aa 1‑300) and a C-terminal TPP-1 dimerization domain (aa 301‑634). There are multiple isoform variants. There is a potential alternate start site at Met132, plus a three aa substitution for aa 43‑634, a 24 aa substitution for aa 317‑634, a three aa substitution for aa 457‑634, and a 16 aa substitution for aa 503‑634. Over aa 159‑316, human POT1 shares 71% aa identity with mouse POT1.
Protection of Telomeres Protein 1
Entrez Gene IDs:
25913 (Human); 101185 (Mouse); 500054 (Rat)
DKFZp586D211; HPOT1; POT1 protection of telomeres 1 homolog; POT1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog (S. pombe); protection of telomeres protein 1
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