Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody Summary
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody (IC0041G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Pref-1 (Preadipocyte Factor 1), also known as DLK1 and FA1, is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins. It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages. Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383). The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity. There are multiple potential splice variants. One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383. Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.
Citation for Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer
Authors: Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri
Cancer Discov, 2020;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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