Human Pref-1/DLK1/FA1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1144A-100
FAB1144A-025
Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry.
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Human Pref-1/DLK1/FA1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1/DLK1/FA1.
Source
Monoclonal Mouse IgG2B Clone # 211309
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1/DLK1/FA1 long isoform
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref‑1/DLK1/FA1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1144A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte Factor 1), also known as DLK1 and FA1, is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Entrez Gene IDs
8788 (Human); 13386 (Mouse)
Alternate Names
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG

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