GDF-15 Antibody [Biotin]

R&D Systems | Catalog # BAF940

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GDF-15 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human GDF-15 (R&D Systems, Catalog # 957-GD)
Ala197-Ile308
Accession # Q99988

Specificity

Detects human GDF-15 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human GDF‑11, recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, and rmGDF-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GDF-15 Antibody [Biotin]

Human GDF-15 ELISA Standard Curve

Human GDF-15 ELISA Standard Curve

Recombinant Human GDF‑15 (Catalog # 957-GD) was serially diluted and captured by Mouse Anti-Human/Primate GDF‑15 Monoclonal Antibody (Catalog # MAB957) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for GDF-15 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human GDF‑15 (Catalog # 957-GD)

Human/Primate GDF-15 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate GDF‑15 Antibody (Catalog # MAB957)
  • Standard: Recombinant Human GDF-15 (CHO-expressed) Protein (Catalog # 957-GD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using BAF940 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

References

  1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  2. Böttner, M. et al. (1999) Gene 237:105.
  3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
  4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Entrez Gene IDs

9518 (Human); 23886 (Mouse); 29455 (Rat)

Gene Symbol

GDF15

UniProt

Q99988

Additional GDF-15 Products

Product Documents for GDF-15 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GDF-15 Antibody [Biotin]

For research use only

Citations for GDF-15 Antibody [Biotin]

Customer Reviews for GDF-15 Antibody [Biotin] (2)

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  • Human/Primate GDF-15 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/08/2019
    This antibody was used as the detection in an ELISA along with MAB957 as the capture antibody. Human serum and plasma samples were measurable.
    GDF-15 Antibody [Biotin] BAF940
  • Human/Primate GDF-15 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/11/2019
    This antibody was used as the pair to MAB957 in an GDF-15 immunoassay.
    GDF-15 Antibody [Biotin] BAF940

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