Detects human Pro-BDNF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human beta -NGF, NT-4, Pro-NT-3 or Pro-NT-4 is observed. In Western blots, 100% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human mature BDNF or recombinant human NT-3 is observed.
Monoclonal Mouse IgG2B Clone # 584412
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Pro-BDNF Ala19-Arg247 Accession # P23560
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immersion fixed paraffin-embedded sections of human brain (spinal cord) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Pro‑BDNF by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Pro‑BDNF Monoclonal Antibody (Catalog # MAB31751) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pro‑BDNF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247 amino acids (aa). Cleavage of an 18 aa signal sequence produces an approximately 35 kDa Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14 kDa BDNF. The propeptides of human and mouse BDNF share 93% aa sequence identity.
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