Human Pro beta -NGF Antibody

Catalog # Availability Size / Price Qty
MAB2562
MAB2562-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human Pro beta -NGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human pro-beta -NGF in Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant mouse (rm) beta -NGF, rr beta -NGF, rhBDNF, rhNT-3, or rhNT-4.
Source
Monoclonal Rat IgG2B Clone # 347112
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human pro-beta -NGF
Glu19-Ala241
Accession # P01138
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Pro beta -NGF. This antibody does not detect mature Recombinant Human  beta -NGF. 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

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Citations for Human Pro beta -NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay
    Authors: S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra
    Research in Veterinary Science, 2022;151(0):156-163.
    Species: Horse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Randomised clinical trial: the analgesic properties of dietary supplementation with palmitoylethanolamide and polydatin in irritable bowel syndrome
    Authors: C Cremon, V Stanghelli, MR Barbaro, RF Cogliandro, L Bellacosa, J Santos, M Vicario, M Pigrau, C Alonso Cot, B Lobo, F Azpiroz, S Bruley des, M Neunlist, D DeFilippis, T Iuvone, S Petrosino, V Di Marzo, G Barbara
    Aliment. Pharmacol. Ther, 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors
    Authors: F Zeng, Z Li, Q Zhu, R Dong, C Zhao, G Li, G Li, W Gao, G Jiang, E Zheng, G Cai, S Moisyadi, J Urschitz, H Yang, D Liu, Z Wu
    Sci Rep, 2017;7(0):41270.
    Species: Mouse
    Sample Types: Saliva
    Applications: Western Blot

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Secondary Antibodies

Reviews for Human Pro beta -NGF Antibody

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Human beta-NGF Antibody
By Anonymous on 06/03/2020
Application: Simple Western Sample Tested: Serum,Synovial fluid Species: Equine

Primary ab: MAB2562: 10ug/ml
Secondary ab: HAF005, diluted 1:50