Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 370104
View all BNP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pro‑BNP
His27-His134
Accession # P16860

Specificity

Detects human Pro‑BNP in direct ELISAs and Western blots. No cross-reactivity with recombinant human BNP (aa 103‑134) is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Pro‑BNP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Pro‑BNP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BNP

In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa signal peptide and a 108 aa Pro-BNP (aa 27-134). Proteolytic digestion of Pro-BNP results in formation of 76 aa amino-terminal NT-proBNP (aa 27-102) and biologically active 32 aa BNP hormone molecule (aa 103-134). Both proBNP and NTpro-BNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Long Name

Brain Natriuretic Peptide

Alternate Names

BNF, NPPB

Entrez Gene IDs

4879 (Human); 18158 (Mouse); 25105 (Rat)

Gene Symbol

NPPB

UniProt

P16860

Additional BNP Products

Product Documents for Human Pro‑BNP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pro‑BNP Antibody

For research use only

Citations for Human Pro‑BNP Antibody

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