Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 499911
View all Proprotein Convertase 9/PCSK9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Proprotein Convertase 9/PCSK9
Arg29-Gln692
Accession # Q8NBP7

Specificity

Detects human Proprotein Convertase 9/PCSK9 in direct ELISAs and Western blots. In Western blots, 10% cross‑reactivity with recombinant mouse PCSK9 is observed and no cross-reactivity with recombinant human (rh) PCSK1 or rhPCSK7 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human Proprotein Convertase 9/PCSK9 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 9/PCSK9 (Catalog # 3888-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Proprotein Convertase 9/PCSK9 (Catalog # 3888-SE)

Reviewed Applications

Read 4 reviews rated 4 using MAB38882 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proprotein Convertase 9/PCSK9

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (aa 1 to 30), a propeptide (aa 31 to 152), and a mature chain (aa 153 to 692) that contains a serine protease domain (aa 161 to 431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non‑covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

References

  1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
  2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)

Gene Symbol

PCSK9

UniProt

Q8NBP7

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Human Proprotein Convertase 9/PCSK9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Proprotein Convertase 9/PCSK9 Antibody

For research use only

Related Research Areas

Obesity
Serine Proteases and Regulators

Customer Reviews for Human Proprotein Convertase 9/PCSK9 Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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1 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Human Proprotein Convertase 9/PCSK9 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Proprotein Convertase 9/PCSK9 Antibody MAB38882
  • Human Proprotein Convertase 9/PCSK9 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human Proprotein Convertase 9/PCSK9 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018
  • Human Proprotein Convertase 9/PCSK9 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018

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