Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Protein Disulfide Isomerase/P4HB Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protein Disulfide Isomerase/P4HB
Asp18-Lys505
Accession # P07237

Specificity

Designed to visualize the expression of P4HB by fluorescence microscopy for staining cells and tissues. Conjugated P4HB antibodies are ideal for immunocytochemistry colocalization studies in the lumen of the endoplasmic reticulum. The unconjugated antibody detects human Protein Disulfide Isomerase/P4HB in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

Protein Disulfide Isomerase/P4HB antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Protein Disulfide Isomerase/P4HB in HeLa Human Cell Line.

Protein Disulfide Isomerase/P4HB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Protein Disulfide Isomerase/P4HB Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4236) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line fixed in formaldehyde

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Protein Disulfide Isomerase/P4HB

P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family. As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4 hydroxylase. This catalyses the hydroxylation of proline in collagen. On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells. Mature human P4HB is 491 amino acids (aa) in length. It contains two TRX domains (aa 25 134 and 368 475) plus an ER retention sequence (aa 505 508). There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18 505, human P4HB shares 94% aa identity with mouse P4HB.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Entrez Gene IDs

5034 (Human); 18453 (Mouse); 25506 (Rat)

Gene Symbol

P4HB

UniProt

P07237

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

For research use only

Related Research Areas

Redox Enzymes

Customer Reviews for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody and earn rewards!

Have you used Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies