Detection of Human Proteinase 3/Myeloblastin/ PRTN3 by Western Blot.
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes (PBL), and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proteinase 3/Myeloblastin/ PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Simple WesternTM.
Simple Western lane view shows lysates of human peripheral blood mononuclear cell tissue, loaded at 0.2 mg/mL. A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proteinase 3/Myeloblastin/PRTN3
Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.
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