Human Protocadherin-1 APC-conjugated Antibody

Name: Human Protocadherin-1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB5899A

Catalog #: FAB5899A Datasheet / COA / SDS

Discontinued Product

FAB5899A has been discontinued.

View all Protocadherin-1 products.

Detection of Protocadherin‑1 in A431 Human Cell Line by Flow Cytometry.

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Human Protocadherin-1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin-1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 648127

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Protocadherin-1
Thr58-Asn162
Accession # Q08174

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Protocadherin‑1 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Protocadherin-1 APC-conjugated Monoclonal Antibody (Catalog # FAB5899A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Protocadherin-1

Protocadherin-1 (PCDH-1; also PC42) is a 150-155 kDa δ1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca⁺⁺-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58-852) plus a 187 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58-844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There is one splice variant that contains a 204 aa substitution for aa 1034-1060 in the cytoplasmic domain. An alternative start site may exist at Met23. Over aa 58-162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1.

Entrez Gene IDs

5097 (Human); 75599 (Mouse); 307481 (Rat)

Alternate Names

cadherin-like 1; Cadherin-like protein 1; FLJ53887; MGC45991; PC42; PC42PCDH42; PCDH1; PCDH42; protocadherin 1 (cadherin-like 1); protocadherin 1; protocadherin 42; Protocadherin1; Protocadherin-1; protocadherin-42