Detection of Protocadherin‑1 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Protocadherin‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5899A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Protocadherin-1 (PCDH-1; also PC42) is a 150-155 kDa δ1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca++-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58-852) plus a 187 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58-844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There is one splice variant that contains a 204 aa substitution for aa 1034-1060 in the cytoplasmic domain. An alternative start site may exist at Met23. Over aa 58-162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1.
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