Human PTH Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB7665AFP680

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 918462
View all PTH Primary Antibodies »

Product Specifications

Specificity

Detects human PTH in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human PTH Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PTH

PTH (Parathyroid Hormone) is a critical hormone in the regulation of Ca++ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle (8, 13).

References

  1. Potts, J.T. (2005) J. Endocrinol. 187:311.
  2. Usatii, M. et al. (2007) Kidney Int. 72:1330.
  3. Scillitani, A. et al. (2011) J. Clin. Invest. 34:23.
  4. Liu, Z. et al. (2010) PLoS Genet. 6:e1001251.
  5. Ben-Dov, I.Z. et al. (2007) J. Clin. Invest. 117:4003.
  6. Carnevale, V. et al. (2010) Bone 47:626.
  7. Schipani, E. et al. (1993) Endocrinology 132:2157.
  8. Jacome-Galarza, C.E. et al. (2011) J. Bone Miner. Res. 26:1207.
  9. Lupp, A. et al. (2010) Eur. J. Endocrinol. 162:979.
  10. Fortunati, D. et al. (2010) Matrix Biol. 29:594.
  11. O’Brien, C.A. et al. (2008) PLoS ONE 3:e2942.
  12. Lombardi, G. et al. (2010) J. Endocrinol. Invest. 33:22.
  13. Zaruba, M.M. et al. (2008) Cardiovasc. Res. 77:722.

Long Name

Parathyroid Hormone

Alternate Names

Parathormone, Parathyrin

Entrez Gene IDs

5741 (Human)

Gene Symbol

PTH

UniProt

P01270

Additional PTH Products

Product Documents for Human PTH Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PTH Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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