Human PTH Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PTH in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 918462
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PTH
    Ser32-Gln115
    Accession # P01270
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PTH by Western Blot. Western blot shows lysates of human parathyroid tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTH at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
PTH in Human Parathyroid Gland. PTH was detected in immersion fixed paraffin-embedded sections of human parathyroid gland using Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTH
PTH (Parathyroid Hormone) is a critical hormone in the regulation of Ca++ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle (8, 13).
  • References:
    1. Potts, J.T. (2005) J. Endocrinol. 187:311.
    2. Usatii, M. et al. (2007) Kidney Int. 72:1330.
    3. Scillitani, A. et al. (2011) J. Clin. Invest. 34:23.
    4. Liu, Z. et al. (2010) PLoS Genet. 6:e1001251.
    5. Ben-Dov, I.Z. et al. (2007) J. Clin. Invest. 117:4003.
    6. Carnevale, V. et al. (2010) Bone 47:626.
    7. Schipani, E. et al. (1993) Endocrinology 132:2157.
    8. Jacome-Galarza, C.E. et al. (2011) J. Bone Miner. Res. 26:1207.
    9. Lupp, A. et al. (2010) Eur. J. Endocrinol. 162:979.
    10. Fortunati, D. et al. (2010) Matrix Biol. 29:594.
    11. O’Brien, C.A. et al. (2008) PLoS ONE 3:e2942.
    12. Lombardi, G. et al. (2010) J. Endocrinol. Invest. 33:22.
    13. Zaruba, M.M. et al. (2008) Cardiovasc. Res. 77:722.
  • Long Name:
    Parathyroid Hormone
  • Entrez Gene IDs:
    5741 (Human)
  • Alternate Names:
    Parathormone; Parathyrin; parathyroid hormone 1; parathyroid hormone; PTH; PTH1
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