Human PTHLH/PTHrP Antibody

Name: Human PTHLH/PTHrP Antibody
Brand: R&D Systems
SKU: AF6884

Catalog #: AF6884 Datasheet / COA / SDS

Discontinued Product

AF6884 has been discontinued.

View all PTHLH/PTHrP products.

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All PTHLH/PTHrP Antibodies

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Human PTHLH/PTHrP Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTHLH/PTHrP in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PTHLH/PTHrP
Ala37-Arg175
Accession # P12272

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PTHLH/PTHrP in Human Colon. PTHLH/PTHrP was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human PTHLH/PTHrP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6884) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTHLH/PTHrP

PTHLH (Parathyroid hormone-like protein; also PTHrP and PLP) is a 17-18 kDa member of the parathyroid hormone family of molecules. It is widely expressed, and serves as an autocrine/paracrine factor for cells of differing tissue type. Although its N-terminus is related to PTH/parathyroid hormone, the molecule, on balance, is not considered a substitute for PTH. Human PTHLH is synthesized as a 177 amino acid (aa) preproprecursor. The preproregion is 36 aa in length and is coupled to a 16 kDa, 141 aa mature molecule (aa 37-177). There are no potential glycosylation sites or identifiable structural domains in the mature region. PTHLH undergoes both alternative splicing and proteolytic processing. Splicing generates two variant isoforms, a 139 aa variant (aa 37-175), and a 20 kDa, 173 aa variant that shows a 34 aa substitution for aa 176-177. Proteolytic processing creates multiple fragments. The three best characterized are PTHLH/PTHrP (1-36) (equivalent to aa 37-72 of the precursor molecule), PTHLH (74-130), and PTHLH (143-175) (otherwise known as osteostatin). These three peptides have distinct functions; the N-terminal peptide is associated with cell growth, the middle peptide with divalent cation transport, and the C-terminal peptide with osteoclast function. Over aa 37-175, human PTHLH shares 86% aa identity with mouse PTHLH.

Long Name

Parathyroid Hormone-like Hormone

Entrez Gene IDs

5744 (Human); 19227 (Mouse); 24695 (Rat)

Alternate Names

BDE2; HHM; MGC14611; Osteostatin; parathyroid hormone-like hormone; Parathyroid hormone-like protein; parathyroid hormone-related protein; PLPosteostatin; PTHLH; PTHR; PTH-related protein; PTHRP; PTH-rP; PTHRPparathyroid hormone-like related protein