Detection of Human PYK2/FAK2 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PYK2/FAK2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PYK2/FAK2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PYK2 (Proline-rich tyrosine kinase 2; also FAK2, RAFTK and CAK beta ) is a 112‑116 kDa member of the FAK subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca++ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. It contains one FERM domain that binds to growth factor receptors (aa 39‑359), a protein kinase domain (aa 425‑683), two Pro‑rich segments that bind SH domains (aa 702‑767 and 831‑869), and one FAT region that interacts with integrins (aa 868‑1009). Phosphorylation of PYK2 at Tyr402/579/580 is associated with changes in activity. There is one splice variant that shows a deletion of aa 739‑780.
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