Human PYK2/FAK2 Antibody

Catalog # Availability Size / Price Qty
AF4589
AF4589-SP

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Detection of Human PYK2/FAK2 by Western Blot.
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Product Details
Citations (1)
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Human PYK2/FAK2 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human PYK2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PYK2
Asn221-Arg411
Accession # Q14289
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PYK2/FAK2 antibody by Western Blot. View Larger

Detection of Human PYK2/FAK2 by Western Blot. Western blot shows lysates of human brain tissue, Daudi human Burkitt lymphoma, and A549 human lung carcinoma cells. PVDF membrane was probed with 1 µg/mL of Human PYK2/FAK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PYK2/FAK2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry PYK2/FAK2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

PYK2/FAK2 in Human Brain. PYK2/FAK2 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human PYK2/FAK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4589) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PYK2/FAK2

PYK2 (Proline-rich tyrosine kinase 2; also FAK2, RAFTK and CAK beta ) is a 112‑116 kDa member of the FAK subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca++ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. It contains one FERM domain that binds to growth factor receptors (aa 39‑359), a protein kinase domain (aa 425‑683), two Pro‑rich segments that bind SH domains (aa 702‑767 and 831‑869), and one FAT region that interacts with integrins (aa 868‑1009). Phosphorylation of PYK2 at Tyr402/579/580 is associated with changes in activity. There is one splice variant that shows a deletion of aa 739‑780.

Long Name
Proline-rich Tyrosine Kinase 2
Entrez Gene IDs
2185 (Human); 19229 (Mouse); 50646 (Rat)
Alternate Names
CADTK; CADTKCAK-beta; CAK beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; EC 2.7.10; FADK2; FAK2; FAK2EC 2.7.10.2; Focal adhesion kinase 2; FRNK; PKB; ppyk2; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; protein-tyrosine kinase 2-beta; PTK; PTK2B protein tyrosine kinase 2 beta; PTK2B; PYK2; PYK2Related adhesion focal tyrosine kinase; RAFTK; RAFTKFADK 2

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Citation for Human PYK2/FAK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells
    Authors: S Li, DM Pritchard, LG Yu
    Cell Death & Disease, 2023-04-13;14(4):268.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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