Human RalB Antibody Summary
Accession # P11234
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human RalB Monoclonal Antibody (Catalog # MAB3920) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (10 ng/lane) were included. A specific band for RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.
Citation for Human RalB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Transcriptional signatures of Ral GTPase are associated with aggressive clinicopathologic characteristics in human cancer.
Authors: Smith, Steven C, Baras, Alexande, Owens, Charles, Dancik, Garrett, Theodorescu, Dan
Cancer Res, 2012;72(14):3480-91.
Sample Types: Whole Cells
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