|Detection of Human RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human RalB Monoclonal Antibody (Catalog # MAB3920) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (10 ng/lane) were included. A specific band for RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.
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