Human RalB Antibody

Catalog # Availability Size / Price Qty
Detection of Human RalB by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human RalB Antibody Summary

Species Reactivity
Detects human, mouse, and rat RalB in Western blots. In Western blots, no cross-reactivity with recombinant human RalA is observed.
Monoclonal Rat IgG2A Clone # 399417
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human RalB
Accession # P11234
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RalB antibody by Western Blot. View Larger

Detection of Human RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human RalB Monoclonal Antibody (Catalog # MAB3920) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (10 ng/lane) were included. A specific band for RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RalB

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name
Ras-like Protein B
Entrez Gene IDs
5899 (Human); 64143 (Mouse); 116546 (Rat)
Alternate Names
GTP binding protein; RalB; RAS-like protein B; ras-related protein Ral-B; v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)

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Citation for Human RalB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcriptional signatures of Ral GTPase are associated with aggressive clinicopathologic characteristics in human cancer.
    Authors: Smith, Steven C, Baras, Alexande, Owens, Charles, Dancik, Garrett, Theodorescu, Dan
    Cancer Res, 2012;72(14):3480-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P


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Reconstitution Buffers

Secondary Antibodies

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