Human/Rat CCR4 PerCP-conjugated Antibody

  
  • Species Reactivity
    Human, Rat
  • Specificity
    Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
  • Source
    Monoclonal Mouse IgG2B Clone # 205410
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR4 transfectants
    Met1-Leu360
    Accession # P51679
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Mouse Anti-Human/Rat CCR4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1567C) or (B) Mouse IgG2B PerCP Isotype Control (Catalog # IC0041C). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR4 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Mouse Anti-Human/Rat CCR4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1567C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR4
CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.
  • Entrez Gene IDs:
    1233 (Human); 12773 (Mouse); 171054 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PerCP-conjugated Isotype Control

Ctrl IC0041C
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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