Detection of CCR4 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human/Rat CCR4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1567R) or (B) Mouse IgG2B Alexa Fluor 647 Isotype Control (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR4, also known as CD194, is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with CLA+, Th-17 and Th-2 type T cells, and with CD4+CD30+ thymocytes and platelets. CCR4 expression has also been reported on mature dendritic cells.
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