Human/Rat CCR4 Alexa Fluor® 647-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
  • Source
    Monoclonal Mouse IgG2B Clone # 205410
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR4 transfectants
    Met1-Leu360
    Accession # P51679
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human/Rat CCR4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1567R) or (B) Mouse IgG2B Alexa Fluor 647 Isotype Control (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR4
CCR4, also known as CD194, is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with CLA+, Th-17 and Th-2 type T cells, and with CD4+CD30+ thymocytes and platelets. CCR4 expression has also been reported on mature dendritic cells.
  • Entrez Gene IDs:
    1233 (Human); 12773 (Mouse); 171054 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis
    Authors: W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia?
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC0041R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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