Human/Rat CCR4 APC-conjugated Antibody

Clone 205410 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB1567A-025
FAB1567A-100
Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Rat CCR4 APC-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
Source
Monoclonal Mouse IgG2B Clone # 205410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CCR4 transfectants
Met1-Leu360
Accession # P51679
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human/Rat CCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB1567A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR4 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Rat CCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB1567A) and Anti-Rat CD3e PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR4

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Entrez Gene IDs
1233 (Human); 12773 (Mouse); 171054 (Rat); 403541 (Canine); 102120178 (Cynomolgus Monkey)
Alternate Names
C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5

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Citations for Human/Rat CCR4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Activated CLL cells regulate IL17F producing Th17 cells in miR155 dependent and outcome specific manners
    Authors: B Jung, G Ferrer, PY Chiu, R Aslam, A Ng, F Palacios, M Wysota, M Cardillo, JE Kolitz, SL Allen, JC Barrientos, KR Rai, N Chiorazzi, B Sherry
    JCI Insight, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010;185(11):6646-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human/Rat CCR4 APC-conjugated Antibody

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Human/Rat CCR4 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100