Human/Rat CCR4 APC‑conjugated Antibody

R&D Systems | Catalog # FAB1567A

Clone 205410 was used by HLDA to establish CD designation
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 205410
View all CCR4 Primary Antibodies »

Product Specifications

Immunogen

Human CCR4 transfectants
Met1-Leu360
Accession # P51679

Specificity

Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Rat CCR4 APC‑conjugated Antibody

Detection of CCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human/Rat CCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB1567A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR4 antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of CCR4 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Mouse Anti-Human/Rat CCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB1567A) and Anti-Rat CD3e PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Rat CCR4 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes and rat splenocytes

Reviewed Applications

Read 1 review rated 4 using FAB1567A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR4

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Alternate Names

CD194

Entrez Gene IDs

1233 (Human); 12773 (Mouse); 171054 (Rat); 403541 (Canine); 102120178 (Cynomolgus Monkey)

Gene Symbol

CCR4

UniProt

P51679

Additional CCR4 Products

Product Documents for Human/Rat CCR4 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Rat CCR4 APC‑conjugated Antibody

For research use only

Citations for Human/Rat CCR4 APC‑conjugated Antibody

Customer Reviews for Human/Rat CCR4 APC‑conjugated Antibody (1)

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  • Human/Rat CCR4 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. <br />Buffer: 2% Human Serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human/Rat CCR4 APC‑conjugated Antibody FAB1567A

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