Human/Rat CCR4 Fluorescein-conjugated Antibody

Clone 205410 was used by HLDA to establish CD designation.
(7 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
  • Source
    Monoclonal Mouse IgG2B Clone # 205410
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR4 transfectants
    Met1-Leu360
    Accession # P51679
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human/Rat CCR4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1567F) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR4
CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.
  • Entrez Gene IDs:
    1233 (Human); 12773 (Mouse); 171054 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome.
    Authors: James E, Gates T, LaFond R, Yamamoto S, Ni C, Mai D, Gersuk V, O'Brien K, Nguyen Q, Zeitner B, Lanteri M, Norris P, Chaussabel D, Malhotra U, Kwok W
    PLoS Pathog, 2016;12(1):e1005375.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
    Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
    J. Immunol., 2009;183(9):5494-501.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission.
    Authors: Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM
    Eur. J. Immunol., 2009;39(5):1288-300.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007;62(2):170-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. CCR4-expressing T cell tumors can be specifically controlled via delivery of toxins to chemokine receptors.
    Authors: Baatar D, Olkhanud P, Newton D, Sumitomo K, Biragyn A
    J. Immunol., 2007;179(3):1996-2004.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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