Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 191704
View all CCR8 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685

Specificity

Detects human and rat CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not the parent cell lines.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Rat CCR8 APC‑conjugated Antibody

Detection of CCR8 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of CCR8 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR8 antibody in Rat Peripheral Blood Cells antibody by Flow Cytometry.

Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry.

Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Rat CCR8 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes and rat peripheral blood cells

Reviewed Applications

Read 3 reviews rated 4.7 using FAB1429A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR8

CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P51685

Additional CCR8 Products

Product Documents for Human/Rat CCR8 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat CCR8 APC‑conjugated Antibody

For research use only

Citations for Human/Rat CCR8 APC‑conjugated Antibody

Customer Reviews for Human/Rat CCR8 APC‑conjugated Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
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Customer Images

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  • Human/Rat CCR8 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Cancer Tissue
    Species: Rat
    Verified Customer | Posted 09/01/2017
  • Human/Rat CCR8 APC-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Ovary tissue
    Species: Rat
    Verified Customer | Posted 08/03/2017
    Band is is clear and beautiful
  • Human/Rat CCR8 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on monocyte FSC-A and SSC-A population. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. <br />Buffer: 2% Human Serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human/Rat CCR8 APC‑conjugated Antibody FAB1429A

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FAQs for Human/Rat CCR8 APC‑conjugated Antibody

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  • Q: Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?

    A: The staining with this antibody is temperature dependent;  it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

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