Human/Rat CCR8 APC-conjugated Antibody

Clone 191704 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB1429A-025
FAB1429A-100
Detection of CCR8 in Human Blood Monocytes by Flow Cytometry.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)

Human/Rat CCR8 APC-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not the parent cell lines.
Source
Monoclonal Rat IgG2B Clone # 191704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685
Formulation
Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR8 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CCR8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR8 antibody in Rat Peripheral Blood Cells antibody by Flow Cytometry. View Larger

Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Entrez Gene IDs
1237 (Human); 12776 (Mouse)
Alternate Names
CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1

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Citations for Human/Rat CCR8 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer
    Authors: S Su, J Liao, J Liu, D Huang, C He, F Chen, L Yang, W Wu, J Chen, L Lin, Y Zeng, N Ouyang, X Cui, H Yao, F Su, JD Huang, J Lieberman, Q Liu, E Song
    Cell Res, 2017-03-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007-04-01;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?

    • The staining with this antibody is temperature dependent;  it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

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Reviews for Human/Rat CCR8 APC-conjugated Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Human/Rat CCR8 APC-conjugated Antibody
By Anonymous on 09/01/2017
Application: Flow Sample Tested: Cancer Tissue Species: Rat

Human/Rat CCR8 APC-conjugated Antibody
By Anonymous on 08/03/2017
Application: WB Sample Tested: Ovary tissue Species: Rat

Band is is clear and beautiful


Human/Rat CCR8 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on monocyte FSC-A and SSC-A population. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100