Human/Rat CCR8 APC-conjugated Antibody

Clone 191704 was used by HLDA to establish CD designation.
(4 citations)
(3 Reviews)
  
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not the parent cell lines.
  • Source
    Monoclonal Rat IgG2B Clone # 191704
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human CCR8
    Met1-Leu355
    Accession # P51685
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 APC‑conjugated Monoclonal Antibody (Catalog # FAB1429A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 APC‑conjugated Monoclonal Antibody (Catalog # FAB1429A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR8
CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.
  • Entrez Gene IDs:
    1237 (Human); 12776 (Mouse)
  • Alternate Names:
    CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer
    Authors: S Su, J Liao, J Liu, D Huang, C He, F Chen, L Yang, W Wu, J Chen, L Lin, Y Zeng, N Ouyang, X Cui, H Yao, F Su, JD Huang, J Lieberman, Q Liu, E Song
    Cell Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  3. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4.7 (Based on 3 reviews)

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Read what people are saying who have used Human/Rat CCR8 APC-conjugated Antibody.

We have 1 review tested in 1 species: Human.
We have 3 reviews tested in 2 applications: Flow Cytometry, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Anonymous 09/01/2017
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Summary

ApplicationFlow Cytometry
Sample TestedCancer Tissue
  Excellent
 WB Anonymous 08/03/2017
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Summary

ApplicationWestern Blot
Sample TestedOvary tissue

Other Experimental Details

Other Experimental DetailsBand is is clear and beautiful
Flow Cytometry Human/Rat CCR8 APC‑conjugated Antibody FAB1429A
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Very Good
 Flow Human Anonymous 10/26/2015
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Image Details
Flow Cytometry Human/Rat CCR8 APC‑conjugated Antibody FAB1429A
Flow Cytometry: Human/Rat CCR8 APC‑conjugated Antibody [FAB1429A]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsGated on monocyte FSC-A and SSC-A population. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

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