Detection of CCR8 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin alpha M/CD11b Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB16991G) and either (A) Rat Anti-Human/Rat CCR8 PE‑conjugated Monoclonal Antibody (Catalog # FAB1429P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR8 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Human/Rat CCR8 PE‑conjugated Monoclonal Antibody (Catalog # FAB1429P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR8 (C-C Chemokine Receptor 8), previously called CKR-L1 or TER1 and designated CD198, is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.