Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 191704
View all CCR8 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685

Specificity

Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Rat CCR8 PE‑conjugated Antibody

Detection of CCR8 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCR8 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin aM/CD11b Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB16991G) and either (A) Rat Anti-Human/Rat CCR8 PE-conjugated Monoclonal Antibody (Catalog # FAB1429P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR8 antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of CCR8 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Rat Anti-Human/Rat CCR8 PE-conjugated Monoclonal Antibody (Catalog # FAB1429P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Rat CCR8 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and rat splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C Chemokine Receptor 8), previously called CKR-L1 or TER1 and designated CD198, is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P51685

Additional CCR8 Products

Product Documents for Human/Rat CCR8 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat CCR8 PE‑conjugated Antibody

For research use only

Citations for Human/Rat CCR8 PE‑conjugated Antibody

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FAQs for Human/Rat CCR8 PE‑conjugated Antibody

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  • Q: Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?

    A: The staining with this antibody is temperature dependent;  it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

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