Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
Monoclonal Rat IgG2B Clone # 191704
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with human CCR8 Met1-Leu355 Accession # P51685
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 20 ng/mL Recombinant Human CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCR8 Antibody.
Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND50 is typically 0.01‑0.05 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a
41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smooth
muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+
T cells, Langerhans cells and neurons. CCL1/TCA3
and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in
length. It contains
a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105
collectively, human and mouse CCR8 share 64% aa sequence identity.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.