Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
Monoclonal Rat IgG2B Clone # 191704
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with human CCR8 Met1-Leu355 Accession # P51685
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 20 ng/mL Recombinant Human CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCR8 Antibody.
Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND50 is typically 0.01‑0.05 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a
41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smooth
muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+
T cells, Langerhans cells and neurons. CCL1/TCA3
and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in
length. It contains
a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105
collectively, human and mouse CCR8 share 64% aa sequence identity.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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