Human CCR8 Antibody

Clone 191704 was used by HLDA to establish CD designation.
(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
  • Source
    Monoclonal Rat IgG2B Clone # 191704
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human CCR8
    Met1-Leu355
    Accession # P51685
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 20 ng/mL Recombinant Human CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCR8 Antibody.

Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND50 is typically
0.01‑0.05 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR8
CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.
  • Entrez Gene IDs:
    1237 (Human); 12776 (Mouse)
  • Alternate Names:
    CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
Sample Type
  1. Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
    Authors: Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, O'Connor D, Lifson JD, Roederer M, Ohlen C
    J. Immunol., 2010;184(1):303-14.
    Species: Primate - Macaca mulatta (Rhesus monkey)
    Sample Type: Whole Cells
    Application: Flow
  2. Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection.
    Authors: Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C
    J. Immunol., 2010;184(1):315-26.
    Species: Primate - Macaca mulatta (Rhesus monkey)
    Sample Type: Whole Cells
    Application: Flow
  3. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006;177(7):4488-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 22  
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Normal Rat IgG Control

Ctrl 6-001-A 7
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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF005 3  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 1  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115
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