Human/Rat CCR8 PerCP-conjugated Antibody
Human/Rat CCR8 PerCP-conjugated Antibody Summary
Accession # P51685
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCR8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1429C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1429C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.
Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?
The staining with this antibody is temperature dependent; it will stain at 4 C and room temperature, however, staining is stronger at 37 C.
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