Human/Rat LRP-4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Rat LRP‑4 by Western Blot.
2 Images
Product Details
Supplemental Products

Human/Rat LRP-4 Antibody Summary

Species Reactivity
Human, Rat
Detects human and rat LRP-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-1B, rhLRP-5, rhLRP-6, and rhLDL R is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human LRP-4
Accession # O75096
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat LRP-4 antibody by Western Blot. View Larger

Detection of Human and Rat LRP‑4 by Western Blot. Western blot shows lysates of human brain (cortex) tissue and rat cortical embryonic neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat LRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRP-4 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry LRP-4 antibody in Human Brainstem by Immunohistochemistry (IHC-P). View Larger

LRP‑4 in Human Brainstem. LRP-4 was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human/Rat LRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. This application has not been tested in rat samples. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-4

LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905).  In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 88% aa identity with mouse LRP‑4.        

Long Name
LDL Receptor-related Protein 4
Entrez Gene IDs
4038 (Human); 228357 (Mouse); 83469 (Rat)
Alternate Names
CLSS; KIAA0816; low density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; LRP10; LRP4; LRP-4; MEGF7LRP10; Multiple epidermal growth factor-like domains 7

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Rat LRP-4 Antibody

There are currently no reviews for this product. Be the first to review Human/Rat LRP-4 Antibody and earn rewards!

Have you used Human/Rat LRP-4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review