Human/Rat LRP-4 Antibody

  
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat LRP-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-1B, rhLRP-5, rhLRP-6, and rhLDL R is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human LRP-4
    Gly18-Ser344
    Accession # O75096
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Rat LRP‑4 by Western Blot. Western blot shows lysates of human brain (cortex) tissue and rat cortical embryonic neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat LRP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRP‑4 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
LRP‑4 in Human Brainstem. LRP‑4 was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human/Rat LRP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. This application has not been tested in rat samples. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-4

LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905).  In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 88% aa identity with mouse LRP‑4.        

  • Long Name:
    LDL Receptor-related Protein 4
  • Entrez Gene IDs:
    4038 (Human); 228357 (Mouse); 83469 (Rat)
  • Alternate Names:
    CLSS; KIAA0816; low density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; LRP10; LRP4; LRP-4; MEGF7LRP10; Multiple epidermal growth factor-like domains 7
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