Human/Rat Neuritin Antibody

(3 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human Neuritin in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neuritin
    Ala28-Gly114
    Accession # Q9NPD7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed cultured rat embryonic hippocampal neurons and rat brain (cortex)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Neuritin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuritin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF283) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuritin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuritin
Neuritin is a 14 kDa GPI-linked molecule that is expressed on developing and differentiating neurons as well as on hepatocytes. Neuritin is upregulated in response to BDNF, NT-3, androgens, and hypoxia, and it promotes neurite extension and arborization. Mature human Neuritin shares 99% and 100% aa sequence identity with mouse and rat Neuritin, respectively.
  • Entrez Gene IDs:
    51299 (Human)
  • Alternate Names:
    dJ380B8.2; neuritin 1; Neuritin; NRN1; NRNMGC44811
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Neuritin Upregulates Kv4.2 ?-subunit of Potassium Channel Expression and Affects Neuronal Excitability by Regulating the Calcium-Calcineurin-NFATc4 Signaling Pathway
    Authors: Jin-Jing Yao
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Neuritin attenuates cognitive function impairments in tg2576 mouse model of Alzheimer's disease.
    Authors: Choi, Yoori, Lee, Kihwan, Ryu, Junghwa, Kim, Hyoun Ge, Jeong, A Young, Woo, Ran-Sook, Lee, Jun-Ho, Hyun, Jin Won, Hahn, Seokyung, Kim, Joung-Hu, Kim, Hye-Sun
    PLoS ONE, 2014;9(8):e104121.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Neuritin mediates nerve growth factor-induced axonal regeneration and is deficient in experimental diabetic neuropathy.
    Authors: Karamoysoyli E, Burnand RC, Tomlinson DR, Gardiner NJ
    Diabetes, 2007;57(1):181-9.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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