Human/Rat Phospho-ATF2 (S490/S498) Antibody

Name: Human/Rat Phospho-ATF2 (S490/S498) Antibody
Brand: R&D Systems
SKU: PPS064

R&D Systems | Catalog # PPS064

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Product Documents

Discontinued Product

PPS064 has been discontinued. View all ATF2 products.

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all ATF2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S490/S498 of ATF2

Specificity

Human and rat ~74 kDa and ~54 kDa splice form of ATF2 phosphorylated at S490/S498 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Human/Rat Phospho-ATF2 (S490/S498) Antibody

Application

Recommended Usage

Immunohistochemistry

1:1000 dilution
Sample: (frozen sections)

Western Blot

1:1000 dilution
Sample: human melanoma cells incubated with varying doses of the radiomimetic drug NCS

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: ATF2

ATF2 (activating transcription factor 2; also CRE-BP1) is a 60 kDa member of the ATF subfamily, basic-region leucine zipper family of DNA binding proteins. It has at least two activities. First, it binds to an octameric palindromic CRE (cAMP response element) 5’-TGACGTCA-3’, and secondary, it shows histone acetyltransferase (HAT) activity towards histones H2B and H4. Its palindromic binding activity depends upon homodimerization, or heterodimerization with either c-Jun or CRE-Bpa. Human ATF2 is 505 amino acids (aa) in length, and contains a C2H2-type zinc finger segment (aa 25-49), a HAT region (aa 112-350), and a basic leucine zipper domain (aa 350-414). There are over a dozen alternate splice forms that, in their entirety, impact all segments of the molecule. ATF2 is phosphorylated at multiple sites, including S62/S90/S112/S340/S368/S490/S498 and T69/T71. The Thr phosphorylations increase transcriptional and HAT activity. Serine phosphorylations at S490/S498 by ATM contribute to DNA damage repair after ionizing radiation. Human ATF2 is greater that 95% aa identical to mouse ATF2 and 100% identical to rat ATF2.