Human/Rat PKC beta 1 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3759X Datasheet / COA / SDS

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AF3759X-100UG

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All PKC beta 1 Antibodies

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Human/Rat PKC beta 1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat PKC beta 1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human PKCδ is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKCÎ²1
Lys607-Val671
Accession # P05771

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PKC beta 1

PKC beta 1 is a 671 aa, 77 kDa member of the PKC subfamily of Ser/Thr family of protein kinases. PKC beta 1 is a Ca²⁺-activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC beta 2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC beta 1 are 100% identical.

Long Name

Protein Kinase C beta 1

Entrez Gene IDs

5579 (Human); 18751 (Mouse); 25023 (Rat)

Alternate Names

PKC beta 1; PKCB;PKCbeta;PKC-beta;PKCI(2);PRKCB;PRKCB1;PRKCB2;Protein kinase C beta type; PRKCB; PRKCB1; PRKCB2