Human/Rat PKC beta 1 Antibody

Catalog # Availability Size / Price Qty
AF3759
AF3759-SP
Human/Rat PKC beta 1 Antibody in Data
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Human/Rat PKC beta 1 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat PKC beta 1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human PKCδ is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PKC beta 1
Lys607-Val671
Accession # P05771
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PKC beta 1
Recombinant Rat PKC beta 1
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry PKC beta 1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

PKC beta 1 in Rat Cortical Stem Cells. PKC beta 1 was detected in immersion fixed rat cortical stem cells using Goat Anti-Human/Rat PKC beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC beta 1

PKC beta 1 is a 671 aa, 77 kDa member of the PKC subfamily of Ser/Thr family of protein kinases. PKC beta 1 is a Ca2+-activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC beta 2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC beta 1 are 100% identical.

Long Name
Protein Kinase C beta 1
Entrez Gene IDs
5579 (Human); 18751 (Mouse); 25023 (Rat)
Alternate Names
PKC beta 1; PRKCB; PRKCB1; PRKCB2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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