Human/Rat PKC beta 1 Antibody

Name: Human/Rat PKC beta 1 Antibody
Brand: R&D Systems
SKU: AF3759
Price: 139 USD
Availability: InStock

R&D Systems | Catalog # AF3759

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PKC beta 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC beta 1
Lys607-Val671
Accession # P05771

Specificity

Detects human and rat PKC beta 1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human PKC delta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Rat PKC beta 1 Antibody

PKC beta 1 in Rat Cortical Stem Cells.

PKC beta 1 was detected in immersion fixed rat cortical stem cells using Goat Anti-Human/Rat PKC beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat PKC beta 1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human neural progenitor cells and immersion fixed rat cortical stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human PKC beta 1
Recombinant Rat PKC beta 1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PKC beta 1

PKC beta 1 is a 671 aa, 77 kDa member of the PKC subfamily of Ser/Thr family of protein kinases. PKC beta 1 is a Ca²⁺-activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC beta 2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC beta 1 are 100% identical.