Human RBX1 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB11499R Datasheet / COA / SDS

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FAB11499R-100UG

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Human RBX1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human RBX1 in Western blots.

Source

Monoclonal Mouse IgG2b Clone # 1076128

Purification

Protein A or G purified

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/RBX1 Complex
Met1-His108
Accession # P62877

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RBX1

RBX1 is a constitutively expressed RING-box protein that interacts with cullins. The human RBX1 gene consists of 5 exons and 4 introns and encodes 108 amino acids with a molecular weight of about 14kda. It is a major component of E3 ubiquitin ligase. A characteristic feature of RBX1 structure is that it contains a RING-H2 finger domain at the carboxyl terminus. RBX1 is overexpressed in many primary tumors and is associated with overall survival and lymph node metastasis. In humans, RBX1 has high expression in the heart, skeletal muscle, and testis with less expression in the brain, lung, kidney, and placenta.

Long Name

Ring-Box 1

Entrez Gene IDs

9978 (Human); 56438 (Mouse); 300084 (Rat)

Alternate Names

BA554C12.1; EC 6.3.2.-; FLJ60363; MGC13357; Protein ZYP; RBX1; Regulator of cullins 1; Ring Box 1; RING box protein 1; RING finger protein 75; ring-box 1; ring-box 1, E3 ubiquitin protein ligase; RING-box protein 1; RNF75; RNF75MGC1481; ROC1E3 ubiquitin-protein ligase RBX1; ZYP Protein