Human ROBO2 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ROBO1, rhROBO3, or rhROBO4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 356001
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ROBO2 isoform 1
    Accession # Q9HCK4.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ROBO2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human ROBO2 PE‑conjugated Monoclonal Antibody (Catalog # FAB3147P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1‑3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31‑504) and three fibronectin (FN) type III domains (aa 522‑826). The cytoplasmic region contains multiple 15‑20 aa long CC (conserved cytoplasmic) motifs (C0‑C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186‑1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22‑285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD shares 98% aa sequence identity with the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1‑ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).

  • References:
    1. Rajagopalan, S. et al. (2000) Neuron 28:767.
    2. Guthrie, S. (2004) Curr. Biol. 14:R632.
    3. Guthrie, S. (2001) Curr. Biol. 11:R300.
    4. Seeger, M. et al. (1993) Neuron 10:409.
    5. Kidd, T. et al. (1998) Cell 92:205.
    6. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
    7. Bashaw, G.J. et al. (2000) Cell 101:703.
    8. Jen, J.C. et al. (2004) Science 304:1509.
    9. GenBank Accession # Q9KCK4.
    10. Isogai, T. et al. (2002) GenBank Accession # AK074780.
    11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
    12. Long, H. et al. (2004) Neuron 42:213.
    13. Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534.
    14. Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.
  • Long Name:
    Roundabout Homolog 2
  • Entrez Gene IDs:
    6092 (Human); 268902 (Mouse); 84409 (Rat)
  • Alternate Names:
    Drosophila) homolog 2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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