S100A7 (also Psoriasin) is an 11‑13 kDa member of the S101 family, EF-hand superfamily of Ca-binding proteins. It is produced by keratinocytes and exists as both an intracellular and extracellular polypeptide. Intercellularly, it binds to E/FABP and may participate in fatty acid metabolism. Extracellularly, it is both monomeric and heterooligomeric and exhibits antibacterial properties. Human S100A7 is 101 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 50‑85), three zinc-binding His residues, one high-affinity Ca-binding site (aa 63‑74) and an overlapping antibacterial sequence (aa 35‑80). There appear to be no meaningful rodent structural orthologs. Human S100A7 is 93% aa identical to human S100A15/7A, and the S100A7 gene has a duplication (S100A7B) that is 54% aa identical to S100A7.
Human S100A7 Antibody
R&D Systems | Catalog # AF4475
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human S100A7
Met1-Gln101
Accession # AAA60210
Met1-Gln101
Accession # AAA60210
Specificity
Detects human S100A7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human S100A8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human S100A7 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human S100A7
Sample: Recombinant Human S100A7
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: S100A7
Long Name
S100 Calcium Binding Protein A7
Alternate Names
PSOR1
Gene Symbol
S100A7
UniProt
Additional S100A7 Products
Product Documents for Human S100A7 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human S100A7 Antibody
For research use only
Related Research Areas
Citations for Human S100A7 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars