Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

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Human S100A8/S100A9 Heterodimer ELISA Standard Curve
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Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL), Human Milk (10 uL), Fecal Extract (50 uL)
0.215 ng/mL
Assay Range
0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Natural and recombinant human S100A8/S100A9 Heterodimer.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human S100A8/S100A9 Heterodimer Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human S100A8/S100A9 Heterodimer in cell culture supernates, tissue lysastes, serum, plasma, saliva, urine, human milk, and fecal extract. It contains E. coli-expressed recombinant human S100A8/S100A9 Heterodimer and antibodies raised against the recombinant factor. Results obtained using natural human S100A8/S100A9 Heterodimer showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human S100A8/S100A9 Heterodimer.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.64 12.1 25.3 6.09 12.6 24.6
Standard Deviation 0.155 0.37 1.14 0.352 0.606 0.792
CV% 2.7 3.1 4.5 5.8 4.8 3.2


The recovery of human S100A8/S100A9 Heterodimer spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 85 79-96
Cell Lysis Buffer (n=3) 94 80-104
Human Milk (n=4) 96 83-103
Urine (n=4) 100 91-113


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human S100A8/S100A9 Heterodimer were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human S100A8/S100A9 Heterodimer ELISA Linearity

Scientific Data

Human S100A8/S100A9 Heterodimer ELISA Standard Curve

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8/S100A9 Heterodimer

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name:
S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Entrez Gene IDs:
6279 (Human)
Alternate Names:
Calprotectin; S100A8/S100A9 Heterodimer
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Citations for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation
    Authors: Z Li, O McGinn, Y Wu, A Bahreini, NM Priedigkei, K Ding, S Onkar, C Lampenfeld, CA Sartorius, L Miller, M Rosenzweig, O Cohen, N Wagle, JK Richer, WJ Muller, L Buluwela, S Ali, TC Bruno, DAA Vignali, Y Fang, L Zhu, GC Tseng, J Gertz, JM Atkinson, AV Lee, S Oesterreic
    Nature Communications, 2022;13(1):2011.
    Species: Human
    Sample Types: Plasma
  2. S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo
    Authors: F Real, A Zhu, B Huang, A Belmellat, A Sennepin, T Vogl, C Ransy, M Revol, R Arrigucci, A Lombès, J Roth, ML Gennaro, F Bouillaud, S Cristofari, M Bomsel
    Nature Communications, 2022;13(1):5956.
    Species: Human
    Sample Types: Tissue Homogenates
  3. Circulating levels of tight junction proteins in multiple sclerosis: Association with inflammation and disease activity before and after disease modifying therapy
    Authors: A Olsson, S Gustavsen, AR Langkilde, TH Hansen, F Sellebjerg, H Bach Sønde, AB Oturai
    Multiple sclerosis and related disorders, 2021;54(0):103136.
    Species: Human
    Sample Types: Serum
  4. Biomarkers of systemic inflammation, soluble IL-2Ralpha and the multiple sclerosis-associated IL2RA SNP rs2104286 in healthy subjects and multiple sclerosis patients
    Authors: S Buhelt, HB Søndergaar, MR Mahler, S Cobanovic, L Börnsen, C Ammitzbøll, AB Oturai, F Sellebjerg
    Multiple sclerosis and related disorders, 2021;54(0):103140.
    Species: Human
    Sample Types: Serum
  5. An Intervention With Michigan-Grown Wheat in Healthy Adult Humans to Determine Effect on Gut Microbiota: Protocol for a Crossover Trial
    Authors: GA Kinney, EN Haddad, LS Garrow, PKW Ng, SS Comstock
    JMIR research protocols, 2021;10(10):e29046.
    Species: Human
    Sample Types: Stool
  6. Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits
    Authors: IA Drosatos, JN Tsoporis, S Izhar, S Gupta, G Tsirebolos, E Sakadakis, AS Triantafyl, A Rigopoulos, D Rigopoulos, LS Rallidis, I Rizos, TG Parker
    Biomolecules, 2021;11(9):.
    Species: Human
    Sample Types: Plasma
  7. Core genes involved in the regulation of acute lung injury and their association with COVID-19 and tumor progression: A bioinformatics and experimental study
    Authors: AV Sen'kova, IA Savin, EV Brenner, MA Zenkova, AV Markov
    PLoS ONE, 2021;16(11):e0260450.
    Species: Human
    Sample Types: Plasma
  8. Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
    Authors: DR Shores, J Fundora, M Go, F Shakeel, S Brooks, SM Alaish, J Yang, CP Sodhi, DJ Hackam, A Everett
    BMC Pediatr, 2020;20(1):250.
    Species: Human
    Sample Types: Serum
  9. Body Mass Index and Calprotectin Blood Level Correlation in Healthy Children: An Individual Patient Data Meta-Analysis
    Authors: A Grand, E Rochette, F Dutheil, D Gozal, V Calcaterra, R Berni Cana, N Cobanoglu, JPM Derikx, G Terrin, B Pereira, E Merlin
    J Clin Med, 2020;9(3):.
    Species: Human
    Sample Types: Plasma
  10. Biomarkers of inflammation and epithelial barrier function in multiple sclerosis
    Authors: A Olsson, S Gustavsen, IC Hasselbalc, AR Langkilde, F Sellebjerg, AB Oturai, HB Søndergaar
    Mult Scler Relat Disord, 2020;46(0):102520.
    Species: Human
    Sample Types: Serum
  11. Toll-like receptor involvement in adolescent scoliotic facet joint degeneration
    Authors: DG Bisson, K Sheng, S Kocabas, E Krock, A Teles, N Saran, JA Ouellet, L Haglund
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. The rationale and design of the personal diet study, a randomized clinical trial evaluating a personalized approach to weight loss in individuals with pre-diabetes and early-stage type 2 diabetes
    Authors: CJ Popp, DE St-Jules, L Hu, L Ganguzza, P Illiano, M Curran, H Li, A Schoenthal, M Bergman, AM Schmidt, E Segal, A Godneva, MA Sevick
    Contemp Clin Trials, 2019;0(0):.
    Species: Human
    Sample Types:
  13. Activation of a TLR9 mediated innate immune response in preeclampsia
    Authors: RD Williamson, FP McCarthy, LC Kenny, CM McCarthy
    Sci Rep, 2019;9(1):5920.
    Species: Human
    Sample Types: Plasma
  14. Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection
    Nat Med, 2016;0(0):.
    Species: Human
    Sample Types: Serum


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Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit
By Mike Ehrhardt on 11/24/2020
Sample Tested: EDTA Plasma

This ELISA performed as expected with low % CV's

Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: Human cells