Human SEC13 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9055-SP
MAB9055-100
Detection  of  SEC13 by Western Blot.
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Human SEC13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SEC13 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1280A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human SEC13
Gly123-Gln322
Accession # P55735
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
0.3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of  SEC13 by Western Blot. View Larger

Detection of SEC13 by Western Blot. Western blot shows lysates of human kidney tissue, human testis tissue, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SEC13 in  HeLa Human Cell Line. View Larger

SEC13 in HeLa Human Cell Line. SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEC13

Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport.  Presenillin-1, which is implicated in early-onset Alzheimer's disease has been shown to interact with Sec13 vesicles.  Additionally, Sec13 has been shown to be a component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the GATOR2 sub-complex of the GATOR complex.

Long Name
Nuclear Pore Complex Protein Nup85
Entrez Gene IDs
6396 (Human); 110379 (Mouse); 297522 (Rat)
Alternate Names
D3S1231E; D3S1231ESEC13L1npp-20; Npp-20; protein SEC13 homolog; SEC13 (S. cerevisiae)-like 1; SEC13 homolog (S. cerevisiae); SEC13; SEC13L1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13R; SEC13-related protein; SEC13RSEC13-like 1 (S. cerevisiae)

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human SEC13 Antibody

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Human SEC13 Antibody
By Anonymous on 12/01/2017
Application: ICC/IF Sample Tested: Human fibroblast Species: Human

Fibroblasts were fixed with 0.4% paraformaldehyde, washed with DPBS and then permeabilized with 0.2% Triton-X/DPBS, washed and blocked with donkey serum. Sec13 diluted 1:100 and applied to the cells for 90 min, unbound antibody removed with DPBS, washed, then donkey-anti-rabb (1:100) applied for 40 mins. Cells were washed, mounted with DapI and viewed with an epi-fluourescent microscope.