Detection of SEC13 by Western Blot.
Western blot shows lysates of human kidney tissue, human testis tissue, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SEC13 in HeLa Human Cell Line.
SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Sec13 is a ubiquitously expressed protein which participates
in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII
vesicle budding complex was first described in the yeast system and was shown
to provide coat proteins in the early secretory pathway. Sec13 is recruited to
endoplasmic reticulum (ER) membranes where it forms a three dimensional
cuboctahedron cage lattice structure by association with Sec31. This vesicular
structure is necessary for ER-Golgi transport. Presenillin-1, which is
implicated in early-onset Alzheimer's disease has been shown to interact with
Sec13 vesicles. Additionally, Sec13 has been shown to be a component of
the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the
GATOR2 sub-complex of the GATOR complex.
Nuclear Pore Complex Protein Nup85
Entrez Gene IDs:
6396 (Human); 110379 (Mouse); 297522 (Rat)
D3S1231E; D3S1231ESEC13L1npp-20; Npp-20; protein SEC13 homolog; SEC13 (S. cerevisiae)-like 1; SEC13 homolog (S. cerevisiae); SEC13; SEC13L1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13R; SEC13-related protein; SEC13RSEC13-like 1 (S. cerevisiae)
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We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.
Immunocytochemistry/Immunofluorescence: Human SEC13 Antibody [MAB9055].
Other Experimental Details
Other Experimental Details
Fibroblasts were fixed with 0.4% paraformaldehyde, washed with DPBS and then permeabilized with 0.2% Triton-X/DPBS, washed and blocked with donkey serum. Sec13 diluted 1:100 and applied to the cells for 90 min, unbound antibody removed with DPBS, washed, then donkey-anti-rabb (1:100) applied for 40 mins. Cells were washed, mounted with DapI and viewed with an epi-fluourescent microscope.