Detection of Human Secretin R by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Secretin R Monoclonal Antibody (Catalog # MAB6387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Secretin R at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Secretin R
SCTR (Secretin receptor) is a 51-64 kDa member of the G-protein coupled receptor 2 family. It is found on alveolar epithelium, bile duct epithelium, pancreatic exocrine duct epithelium, and stomach plus duodenal mucosal epithelium. Mature human SCTR is a 418 amino acid (aa) 7-transmembrane glycoprotein (aa 23-440). It contains a 121 aa N-terminal extracellular region (aa 23-143) plus a 48 aa C-terminal cytoplasmic domain (aa 393-440). There is at least one splice variant that shows a deletion of aa 66-101. SCTR forms homodimers and homooligomers, and heterodimerizes with almost all family B GPCRs (VPAC1; VPAC2; GLP1R; GHRHR; etc.). A splice form of the human Secretin Receptor with deletion of exons 3 and 4 was found expressed in pancreatic adenocarcinomas and cholangiocellular carcinomas, but not in gastrinomas or nonneoplastic pancreas or liver specimens.The deletion in this splice form is predicted to lead to a frame-shift, early truncation, and total absence of transmembrane segments in the receptor protein, whereas the leader sequence responsible for trafficking of the receptor to the cell membrane is preserved. This encoded a 111 aa peptide with its first 43 residues identical to wild-type receptor; but, subsequent to a shift in coding frame and early truncation, the next 68 residues were unique in the transcriptome/proteome. Our antibody was raised against this unique 68aa sequence resulting from putative frame-shift.
Hayes, G.M. et al. (2007) Gastroenterology 133:853.
Korner, M. et al. (2005) Am. J. Pathol. 167:959.
Korner, M. et al. (2006) J. Hepatol. 45:825.
Entrez Gene IDs:
6344 (Human); 81779 (Rat)
pancreatic secretin receptor; SCTR; SCT-R; secretin receptor; SecretinR; SR
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