Human Secretin R Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Secretin R

SCTR (Secretin receptor) is a 51-64 kDa member of the G-protein coupled receptor 2 family. It is found on alveolar epithelium, bile duct epithelium, pancreatic exocrine duct epithelium, and stomach plus duodenal mucosal epithelium. Mature human SCTR is a 418 amino acid (aa) 7-transmembrane glycoprotein (aa 23-440). It contains a 121 aa N-terminal extracellular region (aa 23-143) plus a 48 aa C-terminal cytoplasmic domain (aa 393-440). There is at least one splice variant that shows a deletion of aa 66-101. SCTR forms homodimers and homooligomers, and heterodimerizes with almost all family B GPCRs (VPAC1; VPAC2; GLP1R; GHRHR; etc.). A splice form of the human Secretin Receptor with deletion of exons 3 and 4  was found expressed in pancreatic adenocarcinomas and cholangiocellular carcinomas, but not in gastrinomas or nonneoplastic pancreas or liver specimens.The deletion in this splice form is predicted to lead to a frame-shift, early truncation, and total absence of transmembrane segments in the receptor protein, whereas the leader sequence responsible for trafficking of the receptor to the cell membrane is preserved. This encoded a 111 aa peptide with its first 43 residues identical to wild-type receptor; but, subsequent to a shift in coding frame and early truncation, the next 68 residues were unique in the transcriptome/proteome. Our antibody was raised against this unique 68aa sequence resulting from putative frame-shift. 

Product Datasheets

Related Research Areas

• Cancer Biomarkers
• Digestive Peptide Hormones and Regulators
• Metabolic Peptide Hormones and Regulators
• Pancreatic Endoderm
• Pancreatic Endoderm Cell Markers
• Peptide Hormone G Protein-Coupled Receptors