Human Semaphorin 6A APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) Semaphorin 6A is observed and no cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 3B, or rmSemaphorin 3F is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 169203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6A
    Accession # Q9H2E6
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 6A in Human T Cell Blasts by Flow Cytometry. Human T cell blasts were stained with Mouse Anti-Human Semaphorin 6A APC‑conjugated Monoclonal Antibody (Catalog # FAB1146A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 6A

The Semaphorins constitute a large family of secreted, glycosylphosphatidylinositol (GPI)-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 to 7), have been identified. All Semaphorins contain a conserved 500 amino acid (aa) Sema domain at the amino-terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. They are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2). Class 6 Semaphorins (Sema 6) are transmembrane proteins that share homology with the axon-guiding insect Sema 1A. Human Sema 6A (VIa) cDNA predicts a 1,030 aa protein comprised of an extracellular domain, a transmembrane domain, and a long cytoplasmic tail (3, 4). A secreted form of Sema 6A can repel sympathetic and dorsal root ganglion axons in vitro, indicating a traditional role as an axon guidance signal (5). There is evidence, however, that Sema 6A also functions as a guidance receptor. Sema 6A mutants show a defect in thalamocortical neuron projection that is cell autonomous, and the cytoplasmic tails for Sema 6 contain binding sites for intracellular regulatory molecules such as Evl and Src (6).

  • References:
    1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.
    2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
    3. Zhou, et al. (1997) Mol. Cell Neurosci. 9:26.
    4. Kikuchi, K. et al. (1999) Mol. Cell Neurosci. 13:9.
    5. Xu, X-M. et al. (2000) J. Neurosci. 20:2638.
    6. Leighton, P.A. et al. (2001) Nature 410:174.
  • Entrez Gene IDs:
    57556 (Human); 20358 (Mouse)
  • Alternate Names:
    KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Semaphorin 6A APC-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information