Human Semaphorin 6A APC-conjugated Antibody

FAB1146A has been discontinued. View all Semaphorin 6A products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) Semaphorin 6A is observed and no cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 3B, or rmSemaphorin 3F is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 169203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6A
    Gly19-Thr649
    Accession # Q9H2E6
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 6A in Human T Cell Blasts by Flow Cytometry. Human T cell blasts were stained with Mouse Anti-Human Semaphorin 6A APC‑conjugated Monoclonal Antibody (Catalog # FAB1146A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 6A

The Semaphorins constitute a large family of secreted, glycosylphosphatidylinositol (GPI)-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 to 7), have been identified. All Semaphorins contain a conserved 500 amino acid (aa) Sema domain at the amino-terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. They are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2). Class 6 Semaphorins (Sema 6) are transmembrane proteins that share homology with the axon-guiding insect Sema 1A. Human Sema 6A (VIa) cDNA predicts a 1,030 aa protein comprised of an extracellular domain, a transmembrane domain, and a long cytoplasmic tail (3, 4). A secreted form of Sema 6A can repel sympathetic and dorsal root ganglion axons in vitro, indicating a traditional role as an axon guidance signal (5). There is evidence, however, that Sema 6A also functions as a guidance receptor. Sema 6A mutants show a defect in thalamocortical neuron projection that is cell autonomous, and the cytoplasmic tails for Sema 6 contain binding sites for intracellular regulatory molecules such as Evl and Src (6).

  • References:
    1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.
    2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
    3. Zhou, et al. (1997) Mol. Cell Neurosci. 9:26.
    4. Kikuchi, K. et al. (1999) Mol. Cell Neurosci. 13:9.
    5. Xu, X-M. et al. (2000) J. Neurosci. 20:2638.
    6. Leighton, P.A. et al. (2001) Nature 410:174.
  • Entrez Gene IDs:
    57556 (Human); 20358 (Mouse)
  • Alternate Names:
    KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA
Related Research Areas

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