Serpin A12 (serine proteinase inhibitor-clade A#12; also known as vaspin) is a 45‑50 kDa secreted member of the alpha 1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral adipocytes, and adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown. Human Serpin A12 is 414 amino acids (aa) in length. It contains a 20 aa signal sequence and a 394 aa mature region. A potential "active" inhibitor site lies between Arg363 and Met378. Mature human Serpin A12 shares 61% aa identity with mouse Serpin A12.
Human Serpin A12 Biotinylated Antibody
R&D Systems | Catalog # BAF4410
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A12
Leu20-Lys414
Accession # Q8IW75
Leu20-Lys414
Accession # Q8IW75
Specificity
Detects human Serpin A12 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin F1, and recombinant mouse Serpin E1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Serpin A12 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin A12
Sample: Recombinant Human Serpin A12
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin A12
Alternate Names
OL-64, Vaspin
Gene Symbol
SERPINA12
UniProt
Additional Serpin A12 Products
Product Documents for Human Serpin A12 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Serpin A12 Biotinylated Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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