Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Serpin A12 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A12
Leu20-Lys414
Accession # Q8IW75

Specificity

Detects human Serpin A12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin F1, and recombinant mouse Serpin E1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Serpin A12 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A12, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin A12

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A12

Serpin A12 (serine proteinase inhibitor-clade A#12; also vaspin) is a 45-50 kDa, secreted member of the alpha 1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral adipocytes, and adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin‑resistance. Its molecular target is unknown. Human Serpin A12 is 414 amino acids (aa) in length. It contains a 20 aa signal sequence and a 394 aa mature region. A potential “active” inhibitor site lies between Arg363 and Met378. Mature human Serpin A12 shares 61% aa identity with mouse Serpin A12.

Alternate Names

OL-64, Vaspin

Entrez Gene IDs

145264 (Human); 68054 (Mouse); 191570 (Rat)

Gene Symbol

SERPINA12

UniProt

Q8IW75

Additional Serpin A12 Products

Product Documents for Human Serpin A12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Serpin A12 Antibody

For research use only

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail