Q: NBP2-21669 is primary antibody to Human Serum Albumin. Can this antibody specifically bind to recombinant Human Albumin (rHA)?’

A: This antibody was generated against Human Albumin Serum (Protein accession number P02768.2) and the binding of NBP2-21669 with a recombinant human albumin has not yet been tested. However, technically it should react if your recombinant protein is the same full length sequence and structure as the non-recombinant form.

Human Serum Albumin Antibody

Novus Biologicals | Catalog # NBP2-21669

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all Human Serum Albumin Primary Antibodies »

Product Specifications

Immunogen

Albumin (Human Serum) (Uniprot: P02768)

Reactivity Notes

Cross reactivity against Human Serum Albumin from other tissues and species may occur but has not been specifically determined

Specificity

Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Rabbit anti-Human serum albumin antibody was prepared from monospecific rabbit antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, purified human serum albumin IgG, and human Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Human Serum Albumin Antibody

Western Blot: Human Serum Albumin Antibody [NBP2-21669]

Western Blot: Human Serum Albumin Antibody [NBP2-21669] - Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin.

Applications for Human Serum Albumin Antibody

Application

Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:2000-1:10000

Application Notes

This product is suitable for western blotting. Although not tested, Anti-Human Serum Albumin may be suitable in other applications. Researchers should determine optimal titers for other applications.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Reconstitution

Reconstitute with 2.0 ml deionized water (or equivalent)

Formulation

Lyophilized from Antiserum

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Human Serum Albumin

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

albumin, cell growth inhibiting protein 42, DKFZp779N1935, growth-inhibiting protein 20, PRO0883, PRO0903, PRO1341, serum albumin

Gene Symbol

ALB

UniProt

P02768 (Human)

Additional Human Serum Albumin Products

Product Documents for Human Serum Albumin Antibody

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Certificate of Analysis

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Product Specific Notices for Human Serum Albumin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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