Human Serum Amyloid A1/A2 Antibody Summary
Accession # P0DJI8
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30196).This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Serum Amyloid A1 DuoSet ELISA Kit (Catalog # DY3019-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Serum Amyloid A1/A2 ELISA Standard Curve. Recombinant human Serum Amyloid A1 protein was seriallydiluted 2-fold and captured by Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog #MAB30194) coated on a Clear Polystyrene Microplate (Catalog #DY990).Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30196)was biotinylated and incubated with the protein captured on the plate.Detection of the standard curve was achieved by incubating Streptavidin-HRP(Catalog # DY998)followed by Substrate Solution (Catalog # DY999)and stopping the enzymatic reaction with Stop Solution (Catalog #DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1/A2
Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.
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