Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 370820
View all Serum Amyloid A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SAA1
Arg19-Tyr122
Accession # P02735

Specificity

Detects human Serum Amyloid A1 (SAA1) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse SAA1 and no cross-reactivity with recombinant human (rh) SAA4 is observed. In direct ELISAs, no cross-reactivity with rhSAA2 is observed. 

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Human Serum Amyloid A1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Serum Amyloid A1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Serum Amyloid A1

Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.

Alternate Names

PIG4, SAA1

Entrez Gene IDs

6288 (Human); 20208 (Mouse)

Gene Symbol

SAA1

UniProt

P02735

Additional Serum Amyloid A1 Products

Product Documents for Human Serum Amyloid A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Serum Amyloid A1 Antibody

For research use only

Citations for Human Serum Amyloid A1 Antibody

Customer Reviews for Human Serum Amyloid A1 Antibody

There are currently no reviews for this product. Be the first to review Human Serum Amyloid A1 Antibody and earn rewards!

Have you used Human Serum Amyloid A1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies